核激素受体家族在皮肤鳞状细胞癌细胞中的表达

刘林红，张华，张颖，胡美玲，张斌

1. 重庆市中医院皮肤科，重庆 400011；2. 山东省济南市天桥区疾病预防控制中心，山东济南 250031

Expressions of nuclear hormone receptor superfamily in skin squamous cell carcinoma A431 cells

LIU Lin-hong ,ZHANG Hua ,ZHANG Ying ,HU Mei-ling ,ZHANG Bin

1. Department of Dermatology, Chongqing Hospital of Traditional Chinese Medicine Chongqing 400011,China;2.Centers forDisease Control and Prevention of Tianqiao District,Jinan,Shandong 250031,China

摘要	[目的] 目的通过研究核激素受体家族成员在皮肤鳞癌 A431 细胞中的表达，分析核激素受体家族成员在皮肤鳞癌发生机制中的作用。方法使用 PCR Array 技术分别检测人皮肤角质形成细胞和皮肤鳞状细胞癌细胞中核激素受体成员 mRNA 的相对表达水平。使用 2 - △△ CT 方法计算皮肤鳞状细胞癌细胞中核激素受体 mRNA 的相对变化。结果核激素受体在角质形成细胞以及皮肤鳞状细胞癌细胞中均表达，RXR 二聚体家族成员如 VDR、RXR、RAR、PPAR 以及 LXR 等表达量较高。A431 细胞中多种受体的 mRNA 表达水平较正常角质形成细胞明显升高，尤其是 RXR 二聚体家族成员。结论在皮肤鳞状细胞癌细胞中存在大量核激素受体家族成员，尤其是 RXR 异构二聚体类成员的高表达，这些成员的异常表达可能在肿瘤发生发展中发挥作用，也可能为肿瘤的治疗提供靶点。
关键词	核激素受体 ; 鳞状细胞癌
Abstract：	Objective To detect nuclear hormone receptors expression in skin squamous cell carcinoma A431 cells and analyze its role in the patho-genesis of skin squamous cell carcinoma. Methods PCR Array was used to detect the mRNA expression levels of nuclear hormone receptor membersin keratinocytes and skin squamous cell carcinoma cells. The relative changes of nuclear hormone receptor mRNA in skin squamous cell carcinoma cellswere calculated by 2 - △△ CT . Results Nuclear hormone receptor expression was detected both in keratinocytes and in skin squamous cell carcinoma cells.The expression of RXR dimer family members such as VDR, RXR, RAR, PPAR and LXR was high. The mRNA expression levels of multiple receptors,especially the members of the RXR dimer family, were signifi cantly higher in A431 cells than in normal keratinocytes. Conclusion High expression ofa large number of nuclear hormone receptors, especially the members of the RXR isomeric dimer family might play a role in development or progressionof skin squamous cell carcinoma, which might also provide potential targets of skin tumor therapy.
Keywords	Nuclear hormone receptor ; Squamous cell carcinoma
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